In vivo cardiovascular gene transfer via interventional technique: Initial experimental results
نویسندگان
چکیده
منابع مشابه
Gene Transfer Technique
This article is describing the nine principle techniques for the gene transfection, which are: (1) lipid-mediated method; (2) calcium-phosphate mediated; (3) DEAE-dextran-mediated; (4) electroporation; (5) biolistics (gene gun); (6) viral vectors; (7) polybrene; (8) laser transfection; (9) gene transfection enhanced by elevated temperature, as the references for the researchers who are interest...
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We describe a novel spinal cord (SC) stimulator that is designed to overcome a major shortcoming of existing stimulator devices: their restricted capacity to selectively activate targeted axons within the dorsal columns. This device overcomes that limitation by delivering electrical stimuli directly to the pial surface of the SC. Our goal in testing this device was to measure its ability to phy...
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PURPOSE This study tested whether controlled drying of the cornea increases vector absorption in mouse and rabbit corneas in vivo and human cornea ex vivo, and studied the effects of corneal drying on gene transfer, structure and inflammatory reaction in the mouse cornea in vivo. METHODS Female C57 black mice and New Zealand White rabbits were used for in vivo studies. Donor human corneas wer...
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UNLABELLED This report describes gene transfer in vitro as well as in vivo using cetylated low-molecular mass (600 Da) polyethylenimine (28% of amine groups substituted with cetyl moieties), termed CT-PEI. This compound is hydrophobic and has to be incorporated into liposomes in order to be suitable for gene transfer studies. Serum-induced plasmid DNA degradation assay demonstrated that CT-PEI-...
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We report an electroporation technique for targeting gene transfer to individual cells in intact tissue. Electrical stimulation through a micropipette filled with DNA or other macromolecules electroporates a single cell at the tip of the micropipette. Electroporation of a plasmid encoding enhanced green fluorescent protein (GFP) into the brain of intact Xenopus tadpoles or rat hippocampal slice...
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ژورنال
عنوان ژورنال: Journal of the American College of Cardiology
سال: 1991
ISSN: 0735-1097
DOI: 10.1016/0735-1097(91)91066-n